Journal
VETERINARY PARASITOLOGY
Volume 159, Issue 2, Pages 181-185Publisher
ELSEVIER
DOI: 10.1016/j.vetpar.2008.10.001
Keywords
Europe; Genetic characterization; Genotypes; Ribosomal DNA (rDNA); Second internal transcribed spacer (ITS-2); Sarcoptes scabiei; Wildlife
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Funding
- MURST [2004078701_001]
- SCI Italian Chapter
- Program for Changjiang Scholars and Innovative Research Team in University [IRT0723]
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The present Study examined the relationship among individual Sarcoptes scabiei mites from 13 wild mammalian populations belonging to nine species in four European Countries using the second internal transcribed spacer (ITS-2) Of nuclear ribosomal DNA (rDNA) as genetic marker. The ITS-2 plus primer flanking 5.8S and 28S rDNA (ITS-2+) was amplified from individual mites by polymerase chain reaction (PCR) and the amplicons were sequenced directly. A total of 148 ITS-2+ sequences of 404 bp ill length Were obtained and 67 variable sites were identified (16.59%). UPGMA analyses did not show any geographical or host-specific clustering, and a similar outcome was obtained using population pairwise Fst statistics. These results demonstrated that ITS-2 rDNA does not appear to be suitable for examining genetic diversity among mite populations. (C) 2008 Elsevier B.V. All rights reserved.
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