Journal
VETERINARY PARASITOLOGY
Volume 160, Issue 3-4, Pages 221-224Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2008.10.098
Keywords
Avian erythrocytes; Hemolytic activity; Trichomonas gallinae; Virulence
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Funding
- Veterinary Services, APHIS, USDA [2001-96130032-CA]
- United States Geological Survey, Biological Resources Division, USDI [01ERAG0013]
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The hemolytic activity of 22 Trichomonas gallinae isolates was investigated using an 18 h erythrocyte hemolysis assay which has been shown to correlate with the clinical virulence of T vaginalis. Absorbance of the assay supernatants was measured at 540 nm and expressed as percentage of complete hemolysis. Mean hemolytic activity of the T. gallinae isolates ranged from 3.5% to 53.4% and did not correspond with clinical virulence. The results of this investigation suggest hemolytic activity is not a useful in vitro virulence assay for T gallinae. Published by Elsevier B.V.
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