Dirofilaria immitis and Wolbachia-derived antigens: Its effect on endothelial mammal cells

Antigens of both Dirofilaria immitis and Wolbachia symbiont bacteria are implicated ill the inflammatory pathology of heartworm infection. The aim of the present Study was to compare the stimulatory capacity Of ill Vitro Cultures of vascular endothelial cells by the adult somatic anti, ens of D. immitis (DiSA) and the recombinant form of the Wolbachia surface protein (rWSP). during the first 24 h of stimulation. Our results indicate a different stimulatory activity of the two antigens. Both the DiSA and rWSP stimulate the production of the enzymes responsible of the arachidonic acid metabolism. cyclooxygenase-2, 5-lipoxygenase (5-LO). and leukotriene B-4. Only DiSA stimulates the production of prostaglandin E-2. Related to the adhesion molecules. the DiSA stimulates the expression of intercellular adhesion molecule-1 (ICAM-1) and platelet endothelial cell adhesion molecule-1 (PECAM-1). whereas rWSP stimulates ICAM-1. PECAM-1, and vascular cell adhesion molecule-1 (VCAM-1). Expression of E-cadherin and vascular endothelial growth factor also were stimulated by rWSP. Neither of the two antigens altered the basic physiological mechanisms of endothelial cells. such as cell proliferation, cell cycle, or apoptosis. The biological and pathological significance of these finding, are discussed. (C) 2008 Elsevier B.V. All rights reserved.