Journal
VETERINARY MICROBIOLOGY
Volume 224, Issue -, Pages 70-77Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2018.08.024
Keywords
Classical swine fever virus; E2; E-rns; IgA; IgG; Oral fluid; serum
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Funding
- Pork Checkoff funds through the National Pork Board, Des Moines, Iowa, USA [15-176]
- Chulalongkorn University, Bangkok Thailand [2]
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The objective of this study was to describe oral fluid and serum antibody (IgG, IgA) responses against classical swine fever virus (CSFV) E2 and E-rns proteins in pigs (n = 60) inoculated with a moderately virulent field strain (ALD, n = 30) or a modified live virus vaccine strain (LOM, n = 30). Oral fluid (n = 1391) and serum (n = 591) samples were collected from individually-housed pigs between day post inoculation (DPI) - 14 to 28. Testing revealed the synchronous appearance of E2- and E-rns-specific IgG and IgA antibodies in serum and oral fluids over time, with E2 and E-rns IgG ELISAs providing better diagnostic performance than the IgA ELISAs. Overall the data suggest the feasibility of large-scale, cost-effective screening of populations for CSFV using oral fluid samples. Given the historic issues of cross-reactivity among pestiviruses, future research should focus on the development of CSFV-specific testing platforms for the detection of E2 and/or E-rns IgG in oral fluid, ideally to be used in combination with DIVA vaccines.
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