Journal
VETERINARY MICROBIOLOGY
Volume 172, Issue 3-4, Pages 443-448Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2014.05.034
Keywords
EF1A; Transmissible gastroenteritis coronavirus; Nucleocapsid
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Funding
- National Natural Science Foundation of China [31172350, 31101823]
- Heilongjiang Provincial Natural Science Foundation [JC201118]
- Research Team Program on Scientific and Technological Innovation in Heilongjiang Provincial University [2011TD001]
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Transmissible gastroenteritis coronavirus (TGEV) is an enteropathogenic coronavirus that causes diarrhea in pigs, which is correlated with high morbidity and mortality in suckling piglets. Using the method of GST pull-down with the nucleocapsid (N), N protein was found to interact with swine testes (ST) cells elongation factor 1-alpha (EF1A), an essential component of the translational machinery with an important role in cells. In vitro and in virus-infected cells interaction was then confirmed by co-precipitation. Knockdown of EF1A impairs N protein proliferation and TGEV replication in host cell. It was demonstrated that EF1A plays a role in TGEV replication. The present study thus provides a protein-related information that should be useful for underlying mechanism of coronavirus replication. (C) 2014 Elsevier B.V. All rights reserved.
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