Journal
VETERINARY MICROBIOLOGY
Volume 147, Issue 1-2, Pages 96-102Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2010.06.006
Keywords
Staphylococcus aureus; Borderline methicillin-resistance; Membrane-bound beta-lactamase; Penicillinase; PRPs-hydrolyzing enzyme; Proteomics
Categories
Funding
- Hungarian National Office for Research and Technology [RET-08/2004]
- [ETT 395/KO/03]
Ask authors/readers for more resources
Methicillin and oxacillin-hydrolyzing enzymes of 6 borderline methicillin-resistant and 1 methicillin-resistant Staphylococcus aureus strains isolated from human clinical samples and 4 borderline methicillin-resistant S. aureus strains isolated from bovine mastitis were investigated. As previous studies suggested the involvement of an additional enzyme besides the penicillinase BlaZ in the determination of borderline resistance, we analyzed the expressed extracellular and membrane-bound beta-lactamases with 2-D gel electrophoresis and mass spectrometry. Our analysis showed that the penicillin-hydrolyzing BlaZ alone was responsible for the hydrolysis of both methicillin and oxacillin. All supernatant and membrane fractions contained the same enzyme with slight sequence variations. The size and pl of the proteins were also variable, probably due to spontaneous hydrolysis and/or posttranslational modifications. Interestingly, we found also cytotoxins and other virulence factors in some nitrocefin-hydrolyzing dots, suggesting that those proteins might have a role in the reduction of local antibiotic concentration. (C) 2010 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available