Journal
VETERINARY JOURNAL
Volume 202, Issue 3, Pages 659-662Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2014.09.006
Keywords
Dog; Canine coronavirus; Canine parvovirus; Pantropic; Sequencing
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Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [475223/2013-6]
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Propesq/UFRGS
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Characterization of canine coronavirus (CCoV) strains currently in circulation is essential for understanding viral evolution. The aim of this study was to determine the presence of pantropic CCoV type IIa in tissue samples from five puppies that died in Southern Brazil as a result of severe gastroenteritis. Reverse-transcriptase PCR was used to generate amplicons for sequence analysis. Phylogenetic analysis of the CCoV-Ila strains indicated that they were similar to those found in other countries, suggesting a common ancestor of these Brazilian isolates. This is the first report of pantropic CCoV-II in puppies from Latin America and our findings highlight that CCoV should be included as a differential diagnosis when dogs present with clinical signs and lesions typically seen with canine parvovirus infection. (C) 2014 Elsevier Ltd. All rights reserved.
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