Journal
VETERINARY JOURNAL
Volume 201, Issue 3, Pages 429-432Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2014.05.025
Keywords
Porcine circovirus type 2; Serology; Immunoperoxidase monolayer assay; ELISA
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Funding
- MSD Animal Health
- Universitat Autonoma de Barcelona, Spain
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The aim of this study was to compare and correlate antibody titres against porcine circovirus type 2 (PCV2) in porcine sera (n=1270) obtained by immunoperoxidase monolayer assay (IPMA) with the results of three commercial ELISAs (designated E1, E2 and E3). The correlation between IPMA and ELISA results was excellent (r(2) >= 0.90). Compared to IPMA, E2 had the highest sensitivity (93.0%), followed by E3 (90.1%) and E1 (85.0%); the specificity was 100% for all tests. All three commercial ELIsAs had predictive values similar to those of IPMA and could be used to monitor antibody responses against PCV2 infection and/or vaccination. (C) 2014 Elsevier Ltd. All rights reserved.
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