4.4 Article

Circadian rhythms and the effect of glucocorticoids on expression of the clock gene period1 in canine peripheral blood mononuclear cells

Journal

VETERINARY JOURNAL
Volume 196, Issue 3, Pages 402-407

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.tvjl.2012.10.010

Keywords

Canine; Circadian rhythms; Clock genes; Period1; Glucocorticoids

Funding

  1. Japan Society for the Promotion of Science, Japan
  2. Grants-in-Aid for Scientific Research [25670281, 24658267, 23688040, 23880009] Funding Source: KAKEN

Ask authors/readers for more resources

Circadian rhythms have a periodicity of approximately 24 h and, in mammals, are regulated by clock genes. In this study, expression profiles of clock genes (per1, per2, clock, bmal1 and cry1) were investigated over a single 24 h period by real-time PCR in peripheral blood mononuclear cells (PBMCs) of healthy dogs and canine PBMCs treated in vitro and in vivo with glucocorticoids. Only pen 1 mRNA exhibited daily rhythms in canine PBMCs. Canine PBMCs cultured with dexamethasone in vitro had dose- and time-dependent increases in pen 1 mRNA expression. Intravenous injection of dexamethasone increased expression of pen l in canine PBMCs in vivo. Rhythmic expression of per1 in PBMCs could be used as a molecular marker for monitoring circadian rhythms and the effects of drugs on clock genes in dogs. (C) 2012 Elsevier Ltd. All rights reserved.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available