Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 162, Issue 3-4, Pages 83-95Publisher
ELSEVIER
DOI: 10.1016/j.vetimm.2014.10.002
Keywords
Acute response; Adaptive immunity; Infection; Sea bass
Categories
Funding
- Italian MIUR [PRIN 2008ZWCZ2N, Ritmare SP6_WP4_Az2_UO4_DO1]
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Sea bass were experimentally infected with Listonella anguillarum or Photobacterium damselae subsp. piscicida (Phdp). At 24 and 72 h post-infection, the expression analysis of immune-relevant genes (IL-1 beta, IL-6, IL-8, Hepcidin), the transcriptional level and detection of HSP70, and the quantification of serum iron were investigated in association with the histological analysis and the bacterial recognition in tissues by immunohistochemistry. At 15 days post-infection, the specific antibody response was detected in surviving fish, as well as the transcriptional levels of TcR and BcR sequences. Both experimental infections were characterized by a similar acute response, whereas different histological and immunohistochemistry evidences were observed. In particular, the early reaction appeared suitable for the clearance of L anguillarum, thus limiting the histological lesions, the bacterial dissemination and the further development of acquired immunity in surviving fish. On the contrary, the innate response appeared not enough to resolve the Phdp infection, which was characterized by tissue damage, bacterial widespread and substantial detection of specific humoral immunity in surviving fish, also associated to lymphocytes clonal expansion. Besides the opportunistic conditions involved in fish vibriosis and pasteurellosis, the comparison between these experimental infection models seems to suggest that the rate of development of the acquired immunity is strictly linked to the activation of the host innate response combined to the degree of bacterial virulence. (C) 2014 Elsevier B.V. All rights reserved.
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