Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 145, Issue 1-2, Pages 74-85Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2011.10.012
Keywords
Paratuberculosis; Johne's disease; DNA vaccines; Vaccination
Categories
Funding
- FRIA
- Contractueel Onderzoek FOD-Volksgezondheid, Veiligheid van de Voedselketen en Leefmilieu Convention [RT-07/6]
- Walloon Region
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Mycobacterium avium subsp. paratuberculosis (Map), the etiological agent of chronic enteritis of the small intestine in domestic and wild ruminants, causes substantial losses to livestock industry. Control of this disease is seriously hampered by the lack of adequate diagnostic tools and vaccines. Here we report on the immunogenicity of eight Map specific antigens, i.e. MAP1693c, Ag3, MAP2677c (identified by post-genomic and immunoproteomic analysis of Map secretome) and Ag5, Ag6, MAP1637c, MAP0388 and MAP3743 (identified by bioinformatic in silico screening of the Map genome). Strong, antigen-specific IFN-gamma responses were induced in mice vaccinated with plasmid DNA encoding MAP1693c, MAP1637c, MAP0388 and MAP3743. In contrast, T cell responses in Map infected mice were directed preferentially against Ag5 and to a lesser extent against MAP3743. None of the tested DNA vaccines conferred protection against subsequent challenge with Map. (C) 2011 Elsevier B.V. All rights reserved.
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