Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 147, Issue 1-2, Pages 97-103Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2012.03.013
Keywords
Avian; Cytokine; Bioactivity; Cross-reactivity
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Funding
- British Poultry Federation Research Association
- BBSRC
- Biotechnology and Biological Sciences Research Council [BBS/E/D/20320000, BBS/E/D/20231760, BBS/E/D/20231759] Funding Source: researchfish
- BBSRC [BBS/E/D/20231759, BBS/E/D/20231760, BBS/E/D/20320000] Funding Source: UKRI
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The cDNAs of two turkey cytokines, interleukin (IL)-10 and IL-13, were cloned using oligonucleotide primers designed from their chicken orthologues. The coding regions of the chicken and turkey genes are highly conserved, with IL-10 and IL-13 exhibiting 94.1% and 90% nucleotide and 92% and 79.9% amino acid identity respectively. Both showed consistent mRNA expression in turkey lymphoid and gut tissues. Expression in non-lymphoid tissues was more variable but generally highest in the skin and trachea. Recombinant turkey IL-10 was expressed and bioactivity demonstrated by inhibition of IFN-gamma synthesis from activated splenocytes. Chicken and turkey IL-10 cross-reacted in functional assays. (C) 2012 Elsevier B.V. All rights reserved.
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