Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 139, Issue 2-4, Pages 245-249Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2010.08.007
Keywords
Fasciola hepatica; Tegument; Macrophage; Arginase; 2D gel electrophoresis
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Funding
- EU Commission [FOOD-CT-2005-02305-DELIVER]
- TUBITAK (The Scientific and Technological Research Council of Turkey)
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Fasciola hepatica, the liver fluke, is a highly evolved endo-parasite that uses various mechanisms to evade the host immune system. The immunosuppressive capabilities of the parasite's excretory/secretory products have been well demonstrated by previous independent studies. However, the role of the parasite's tegument in the immune responses remains to be investigated. In this study, the effect of the tegument and other fractions of adult F. hepatica (excretory/secretory, liver fluke homogenate and liver fluke homogenate without tegument) in the activation of naive macrophages in vitro was investigated using an ovine model. In addition, an immunoproteomic approach was used to investigate the characteristics of humoral antibody responses developed in sheep against the tegument fraction. The results indicated significantly increased arginase expression in macrophages incubated with the tegument and excretory/secretory fractions. Two dimensional gel electrophoresis of the tegument demonstrated approximately 100 protein spots, with only four of these spots were highly reactive with the positive serum as determined by 2-DE immunoblotting. These results give a preliminary indication that the liver fluke tegument may play role in avoiding hosts' protective immune responses against itself. (C) 2010 Elsevier B.V. All rights reserved.
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