Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 129, Issue 1-2, Pages 57-65Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2008.12.011
Keywords
Classical swine fever virus (CSFV); Alphavirus replicon vector; Cell-mediated immunity (CMI); VP22
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Funding
- National 863 Project of China [2006AA10A204]
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We have previously shown that an alphavirus replicon-vectored DNA vaccine (pSFV1CS-E2) encoding the E2 glycoprotein of classical swine fever virus (CSFV) completely protected the immunized pigs from lethal challenge. These animals developed only low or moderate level viral-specific antibody titers before challenge, implying that cell-mediated immunity (CMI) probably played an important role in the protective immunity against CSFV conferred by the DNA vaccine. In this study, the CMI induced by pSFV1CS-E2 and its derivative pSFV1CS-E2-UL49 encoding a fusion protein of CSFV E2 and pseudorabies virus (PRV) VP22 was evaluated in a mouse model by lymphoproliferation assays based on USE or WST-8, intracellular cytokine staining, and cytokine ELISA. The results showed that both vaccines induced CSFV-specific lymphoproliferative responses and cytokine production, and pSFV1CS-E2-UL49 induced stronger lymphoproliferative responses and higher cytokine levels than pSFV1CS-E2. These findings suggest that the alphavirus replicon-delivered DNA vaccines are capable of inducing CMI, and PRV VP22 is able to enhance the immunogenicity of the co-delivered antigen. (c) 2008 Elsevier B.V. All rights reserved.
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