Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 122, Issue 1-2, Pages 46-56Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2007.10.013
Keywords
West Nile virus; horse; IgM; ELISA; monoclonal antibody
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West Nile virus (WNV) is a zoonotic pathogen of global importance. In horses with neurological signs, detection of WNV-specific immunoglobulin M (lam) in serum is widely used to identify clinical cases of WNV encephalitis. Here, we describe the development of two monoclonal antibodies (mAbs) to equine lam which were used in a WNV IgM-specific enzyme-linked immunosorbent assay (ELISA). Their performance was compared to an established assay based on polyclonal anti-lam. Check test serum samples from the National Veterinary Service Laboratory (NVSL) were used to evaluate the performance of the three anti-IgM antibodies. The anti-IgM 1-22 mAb correctly identified all NVSL samples. Both the polyclonal antibody and monoclonal anti-IgM 2B-63 identified eight out of ten samples correctly. The three assays were then compared using serum samples from clinically healthy animals (n = 33) and horses with neurological signs (n = 2 1). High Spearman rank correlations (0.76-0.86) were found among the ELISA results. Inter-test agreements (weighted kappa) for assay interpretation resulted in strong agreement (0.95) of the results obtained by the mAbs and moderate agreements when monoclonal and polyclonal anti-IgM-based assays were compared. To determine the analytical sensitivities of anti-WNV IgM detection, serial dilutions of WNV IgM-positive serum samples were analyzed. The highest sensitivity was obtained by using the anti-IgM 1-22 mAb to capture lam from equine serum. In conclusion, the use of monoclonal anti-IgM antibodies can improve the sensitivity of lam detection in the acute phase of WN disease. (C) 2007 Elsevier B.V. All rights reserved.
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