Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 126, Issue 1-2, Pages 131-141Publisher
ELSEVIER
DOI: 10.1016/j.vetimm.2008.06.013
Keywords
Caseous lymphadenitis; Goats; C. pseudotuberculosis; CpG ODN; Vaccine
Categories
Funding
- Laboratory Of Immunology of Health Sciences Institute of Federal University of Bahia
- Scientific and Technological Development Fund
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Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis, a disease that affects goats and sheep, and can cause severe economic losses. In this study, four different antigenic extracts were obtained from the attenuated strain T 1, which was isolated in the state of Bahia (Brazil). Forty-four Caninde breed goats were divided in five groups, each receiving a different antigen Solution and saline buffer as a control. The humoral response was monitored through the identification of specific IgG by indirect ELISA and Western Blotting, and the production of IFN-gamma was followed in order to observe the activation of cellular response. After twelve weeks of antigen inoculation, the animals were challenged with 2 x 10(5) CFU of a wild strain, also isolated in Bahia, and necropsy was performed on all animals twelve weeks afterwards. It was observed that the attenuated bacteria gave a protection of 33.3%, in addition to the weak humoral response elicited. Animals inoculated with secreted antigen associated with Freund's incomplete adjuvant and oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) showed a strong humoral response, but this inoculation could not prevent the spread of challenge bacteria in the majority of animals. These results demonstrate the immunogenic potential of the attenuated T I strain in the development of a vaccine against caseous lymphadenitis in goats. (C) 2008 Elsevier B.V. All rights reserved.
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