Journal
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
Volume 121, Issue 3-4, Pages 199-205Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetimm.2007.09.010
Keywords
probiotic; fish disease; acute phase response; innate immunity; proteomics
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The response of rainbow trout (Oncorhynchus mykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomic analysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbow trout serum following feeding with probiotics Aeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin. (c) 2007 Elsevier B.V. All rights reserved.
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