Journal
VETERINARNI MEDICINA
Volume 51, Issue 3, Pages 89-92Publisher
CZECH ACADEMY AGRICULTURAL SCIENCES
DOI: 10.17221/5525-VETMED
Keywords
ultrastructure; iron nanoparticles; cultivation; animal model
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Mononuclear cells from rabbit bone marrow were cultured for 14 days in cell-free medium for hematopoietic cells together with iron oxid nanoparticles, and then they were processed by technique for free cells for TEM ( transmission electron microscopy). Staining with turnbull blue was used for the detection of iron using a light microscope. It was shown that iron nanoparticles were incorporated into the cytoplasm of mononuclear cells during 14 days cultivation. Here they were localized within different sized vacuoles with distinct membranes.
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