Journal
VETERINARNI MEDICINA
Volume 55, Issue 11, Pages 551-560Publisher
CZECH ACADEMY AGRICULTURAL SCIENCES
DOI: 10.17221/3012-VETMED
Keywords
bromelain; chondrocyte; dog; cysteine proteinase
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Funding
- National Centre for Genetic Engineering and Biotechnology (BIOTEC)
- Thailand Ministry of Science and Technology
- Thailand Excellence Centre for Tissue Engineering, Department of Biochemistry, Faculty of Medicine, Chiang Mai University
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The purpose of this study was to determine the effect of bromelain treatment on canine articular chondrocytes in vitro. This research evaluated cell viability, levels of apoptotis and mitotis, proteoglycan concentrations and the expression of certain genes. Chondrocytes were exposed to 50 mu g/ml bromelain for 4, 16 and 32 h. The rate of apoptotis in the treatment groups was significantly lower than in the control groups that were incubated with media only (P < 0.05); and the mitotic rate in treatment groups was significantly higher than in the control groups (P < 0.05), at all durations of exposure. The effect of bromelain on gene expression was measured by the real-time PCR technique. It was found that bromelain significantly decreased (P < 0.05) TIMP-1 and MMP-3 expression. These experimental bromelain treatments have shown positive results, and have increased the basic knowledge in regard to the healing and modulation of osteoarthritis, prior to the general use of bromelain in clinical practice.
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