Journal
VACCINE
Volume 32, Issue 33, Pages 4111-4116Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2014.05.071
Keywords
Epitope; Mouse; TLR-4; TLR-9; OVA; VSV; ELISPOT
Categories
Funding
- United States Government
- Microsoft Research
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We describe a vaccine delivery mechanism consisting of a synthetic, non-living vector of large d,I poly(lactic-co-glycolic) acid (PLGA) microspheres that carry specific cytotoxic T lymphocyte (CTL) epitopes. We demonstrate in mice that it can be used to elicit substantial interferon gamma ELISPOT responses to more than one specific epitope in the same individual. Our data suggest that a superior adjuvant configuration for the formulation is to place a TLR-9 agonist CpG inside the microsphere and a TLR-4 agonist MPLA in the injectate solution. This finding contrasts with the observations of others. Our approach provides a means to elicit immune responses efficiently to select epitopes, which may be important for an effective vaccine against HIV. (C) 2014 The Authors. Published by Elsevier Ltd.
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