Journal
VACCINE
Volume 31, Issue 15, Pages 1916-1923Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2013.02.020
Keywords
Probiotics (LGG+Bb12); Colonization; Antibody responses; Rotavirus vaccine; Colostrum/milk; TGF beta
Categories
Funding
- NCCAM, NIH [R21 AT004716]
- NIAID, NIH [R01 A1099451]
- Ohio Agricultural Research and Development Center (OARDC) of The Ohio State University
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Breast milk (colostrum [col]/milk) components and gut commensals play important roles in neonatal immune maturation, establishment of gut homeostasis and immune responses to enteric pathogens and oral vaccines. We investigated the impact of colonization by probiotics, Lactobacillus rhamnosus GG (LGG) and Bifidobacterium lactis Bb12 (Bb12) with/without col/milk (mimicking breast/formula fed infants) on B lymphocyte responses to an attenuated (Att) human rotavirus (HRV) Wa strain vaccine in a neonatal gnotobiotic pig model. Col/milk did not affect probiotic colonization in AttHRV vaccinated pigs. However, unvaccinated pigs fed col/milk shed higher numbers of probiotic bacteria in feces than non-col/milk fed colonized controls. In AttHRV vaccinated pigs, col/milk feeding with probiotic treatment resulted in higher mean serum IgA HRV antibody titers and intestinal IgA antibody secreting cell (ASC) numbers compared to col/milk fed, non-colonized vaccinated pigs. In vaccinated pigs without col/milk, probiotic colonization did not affect IgA HRV antibody titers, but serum IgG HRV antibody titers and gut IgG ASC numbers were lower, suggesting that certain probiotics differentially impact HRV vaccine responses. Our findings suggest that col/milk components (soluble mediators) affect initial probiotic colonization, and together, they modulate neonatal antibody responses to oral AttHRV vaccine in complex ways. (C) 2013 Elsevier Ltd. All rights reserved.
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