Journal
VACCINE
Volume 30, Issue 48, Pages 6777-6782Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.09.021
Keywords
Plague; Y. pestis; Gold nanoparticle; Vaccine; Carbodiimide
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Funding
- Western Regional Center for Excellence, USA [U54 AI057156]
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The efficacy of 15 nm gold nanoparticles (AuNP) coated with Yersinia pestis F1-antigen, as an immunogen in mice, has been assessed. The nanoparticles were decorated with F1-antigen using N-hydroxysuccinimide and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride coupling chemistry. Mice given AuNP-F1 in alhydrogel generated the greatest IgG antibody response to F1-antigen when compared with mice given AuNP-F1 in PBS or given unconjugated F1-antigen in PBS or alhydrogel. Compared with unconjugated F1-antigen, the IgG2a response was enhanced in mice dosed with AuNP-F1 in PBS (p < 0.05) but not in mice immunised with AuNP-F1 in alhydrogel. All treatment groups developed a memory response to F1-antigen, the polarity of which was inflenced by formulation in alhydrogel. The sera raised against F1-antigen coupled to AuNPs was able to competitively bind to rF1-antigen, displacing protective macaque sera. (C) 2012 Elsevier Ltd. All rights reserved.
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