Journal
VACCINE
Volume 30, Issue 11, Pages 1911-1916Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2012.01.026
Keywords
HIV-1; Gp41; Epitopes; Sequential oligopeptide carriers; Immunogens; Neutralizing antibodies
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Funding
- Deutsche Forschungsgemeinschaft [GRK1172]
- Federal Ministry of Health
- Ministry of Higher Education, Research and the Arts from the state of Hessen
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Peptide mimics of epitopes for pathogen-specific antibodies present in patient sera can be selected based on the phage display technology. Such mimotopes potentially represent vaccine candidates in case they are able to induce neutralizing antibodies upon vaccination. Here we analyze the immunogenicity of different conjugates of epitope EC26-2A4 localizing to the membrane proximal external region (MPER) of the HIV-1 transmembrane protein gp41. The EC26-2A4 epitope, which overlaps with that of the broadly neutralizing monoclonal antibody (mAb) 2F5, was coupled to sequential oligopeptide carriers (SOC) or to palmitoyl acid for better immunogenicity. Upon prime-boost immunizations of mice, the peptide conjugates induced EC26-2A4 specific antibodies in all settings and mice sera neutralized HIV-1SF162.LS in standardized neutralization assays. Thus, the EC26-2A4 MPER epitope represents a promising vaccine candidate for further analysis in larger animals with respect to the breadth of the neutralizing antibodies induced. (C) 2012 Elsevier Ltd. All rights reserved.
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