Journal
VACCINE
Volume 29, Issue 47, Pages 8549-8556Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.09.029
Keywords
Swine influenza; DNA vaccine; Chitosan nanoparticles; Complex coacervation; Oral gene delivery
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Funding
- National Natural Science Foundation of China [31072119, 31000773]
- Key Scientific and Technological Planning Project of Harbin [2009AA6CN125]
- Innovation Foundation of Harbin [2010RFQXN091]
- Heilongjiang University [Hdtd2010-17]
- Heilongjiang Postdoctoral Foundation [LBH-Q07109]
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Preparation conditions of a DNA vaccine against swine influenza encapsulated in chitosan nanoparticies were determined. The nanoparticles were prepared according to a complex coacervation method using chitosan as a biodegradable matrix forming polymer. Under the preparation conditions, chitosan nanoparticles containing the DNA vaccine were produced with good morphology, high encapsulation rate and high stability. Transfection test indicated that the vaccine could be expressed as an antigen in cells, and maintained good bioactivity. In addition, better immune responses of mice immunized with the chitosan nanoparticles containing the DNA vaccine were induced and prolonged release of the plasmid DNA was achieved compared to the DNA vaccine alone. These results laid a foundation for further development of DNA vaccines in nanoparticles before ultimate industrial application. (C) 2011 Elsevier Ltd. All rights reserved.
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