Journal
VACCINE
Volume 29, Issue 47, Pages 8619-8623Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.09.016
Keywords
Toxoplasma gondii; Multiple antigenic peptide; Vaccine
Categories
Funding
- National Special Research Programs for Non-profit Trades (Agricultures) [200903036-02, 200803017]
- National Beef Cattle and Yak Industrial Technology System [nycytx-38]
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To develop a multiple antigenic peptide (MAP) vaccine against toxoplasmosis, tri-epitope MAP constructs were made in dimeric fashion. The constructs included one B-cell and two 1-cell epitopes derived from Toxoplasma gondii antigens (SAG1, GRA4 and GRA1) situated in tandem through the GGG spacer sequence, with the latter positioned adjacent to a polylysine core. Immunization of BALB/c and Kunming mice with the MAP construct in Freund's adjuvant induced not only humoral immunes response but cellular responses. These responses were accompanied by significant levels of splenocyte proliferation and interferon gamma (IFN-gamma) in vitro. After lethal challenge, vaccinated mice had increased survival time in comparison to unvaccinated controls. Our data demonstrate that a MAP construct could trigger strong humoral and cellular responses against T. gondii, and that this MAP is a vaccine candidate worth further development. (C) 2011 Elsevier Ltd. All rights reserved.
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