Journal
VACCINE
Volume 29, Issue 52, Pages 9611-9617Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2011.10.053
Keywords
Glycyrrhetinic acid liposome; Lymphocyte proliferation; Lymphocyte subpopulations; Serum antibody titer; Immunoglobulins
Categories
Funding
- National Natural Science Foundation of China [30901085]
- Fundamental Research Funds for the Central Universities [KYZ201117]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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The aim of this study is to investigate whether the activity of inducing immune response of glycyrrhetinic acid (GA) could be enhanced after GA was encapsulated with liposome. Three hundred and fifty 14-day-old chickens were randomly assigned to 7 groups and vaccinated with Newcastle disease (ND) vaccine. Simultaneously, the chickens in experimental groups were injected with the glycyrrhetinic acid liposome (GAL) at three doses. GA and blank liposome, respectively. The activity of serum antibody titer, concentrations of immunoglobulins G (IgG) and immunoglobulins M (IgM), lymphocytes proliferation, the proportions of lymphocyte subpopulations (CD4(+) and CD8(+)) was determined after vaccination. GAL was evaluated for inducing humoral immunity and cellular immunity in chicken against Newcastle disease (ND) vaccine. The results showed that GAL not only could significantly enhance the antibody titers. IgG and IgM in ND vaccine immunized chicken, but also significantly promote the lymphocyte proliferation and the proportions of CD4(+) and CD8(+)., as comparison with GA, BL and VC control groups. Moreover, the effects appear a dose-dependent manner and a time-dependent manner. These indicated that GAL could significantly promote the activation potential of humoral immunity and cellular immunity in chicken and present certain dose-effect and time-effect relationships. The formulations of GA and liposome can further enhance the immune response against ND vaccine compared with the adjuvant alone. (C) 2011 Elsevier Ltd. All rights reserved.
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