Journal
VACCINE
Volume 28, Issue 51, Pages 8197-8202Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2010.09.032
Keywords
Antigenicity reduction; Coated antibody; Mannosamine-biotin adduct; Passive vaccination; Snake venom
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Passive immunization with cross-species antibodies triggers the patient's immune response, thereby preventing repeated treatment. Mannosamine-biotin adduct (MBA) has been described as a masking agent for immunogenic reduction and here, the immunogenicity and biological activity of MBA-coated horse anti-viper venom (hsIgG) were compared to those of uncoated or PEGylated hsIgG. In in vitro tests, hsIgG binding was not affected by MBA conjugation. The immune response to hsIgG-MBA was about 8-fold and 32-fold lower than to PEG-coated and uncoated hsIgG, respectively. In vivo, hsIgG-MBA showed efficient venom-neutralization activity. We thus demonstrate the feasibility of using MBA as a masking agent for passive immunization with cross-species antibodies. (c) 2010 Elsevier Ltd. All rights reserved.
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