Journal
VACCINE
Volume 28, Issue 7, Pages 1887-1892Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.11.049
Keywords
Mumps virus; Cytopathic effect; Virus potency estimate
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Funding
- Ministry of Science, Education and Sports of the Republic of Croatia [TP-05/0021-02, 021-0212432-3123]
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The two most commonly used methods for the determination of a virus potency are plaque assay and 50% cell culture infective dose (CCID(50)) assay, both based on cytopathic effect observation. We compared the potency estimates obtained by plaque and CCID(50) assays for nine mumps virus strains that produce different cytopathic effects in Vero cells. The ratios of CCID(50) and plaque assay quantification results differed for different strains and were in a range of 0.66-10, indicating that quantification results for some mumps virus strains are almost identical regardless of whether CCID(50) or plaque method is used. while the potency estimates of other strains strongly depend on the choice of the assay. (C) 2009 Elsevier Ltd. All rights reserved.
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