Journal
VACCINE
Volume 28, Issue 5, Pages 1148-1155Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.11.042
Keywords
Newcastle disease; Interleukin-18; Interferon-gamma; Nitric mono-oxide; Hemagglutination inhibition; Chicken
Categories
Funding
- Council of Agriculture in Taiwan [96AS-14.6.1-BQ-B6, 97AS-14.6.1-BQ-B6, 98AS-14.6.1-13Q-135]
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Interleukin-18 (IL-18) can induce interferon-gamma (IFN-gamma) production and promoteTh1 immunity, and hence, it modulates immune functions. In the present study, the in vitro and in vivo immunomodulatory activities of full length or mature chicken IL-18 expressed in a prokaryotic expression system (pCHIL18-F and pCHIL18-M, respectively) and chicken IL-18 expressed in a eukaryotic expression system (euCHIL18) were examined. Results showed that pCHIL18-F, pCHIL18-M and euCHIL18 significantly enhanced IFN-gamma mRNA expression in chicken splenocytes, which successfully increased IF-gamma-induced nitric oxide (NO) synthesis by macrophages, Vaccination with cell-cultured Newcastle disease vaccine (NDTC) co-administrated with pCHIL18-F, pCHIL18-M or euCHIL18 resulted in significant increments of hemagglutination inhibition (HI) titers, cell proliferation of peripheral blood mononuclear cells (PBMC), and ratios of CD8(+) to CD4(+) in chickens compared with inoculation of PBS or NDTC alone. Thus, full length and mature chicken IL-18 expressed using a prokaryotic system and using a eukaryotic system showed equivalent in vitro and in vivo biological activities, and all forms effectively enhanced cell-mediated and humoral immunity, suggesting possible future use as a potential adjuvant in chicken NDTC vaccine production. (C) 2009 Elsevier Ltd. All rights reserved.
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