Journal
VACCINE
Volume 28, Issue 5, Pages 1226-1233Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.11.021
Keywords
T cell priming; Mucosal vaccines; Vaccine vectors; Streptococcus gordonii
Categories
Funding
- Commission of the European Communities, Sixth Framework Programme [LSHP-Cr-2003-503240]
- Mucosal Vaccines for Poverty-Related Diseases (MUVAPRED)
- European Vaccines and Microbicides Enterprise (EUROPRISE) [037611]
- Istituto Superiore di Sanita [45G.27]
Ask authors/readers for more resources
Generation of primed T cells is crucial for the development of optimal vaccination strategies. Using a TCR-transgenic CD4(+) and CD8(+) T cell adoptive transfer model, we demonstrate that a single nasal immunization with recombinant Streptococcus gordonii induces antigen-specific primed T cells in lymph nodes draining the genital and intestinal tracts with about 80% of CD4(+) and 50% of CD8(+) proliferating cells. T cell clonal expansion was also observed in cervical lymph nodes, draining the immunization site, and in the spleen. The modulation of CD44 and CD45RB marker expression indicated that proliferating T cells were activated. Proliferation in distal mesenteric and iliac lymph nodes and in the spleen was observed 5 days after nasal immunization, while in draining cervical lymph nodes proliferation peaked already at day 3. The division profile of transgenic T cells observed in iliac and mesenteric lymph nodes was discontinuous, showing the lack of early cell divisions. The kinetics of T cell clonal expansion, the discontinuous division profile and the modulation of migration markers such as CD62L suggest that activated antigen-specific T cells disseminate from the immunization site to distal intestinal and genital tracts. These data demonstrate the efficacy of nasal immunization with recombinant S. gordonii in eliciting CD4(+) and CD8(+) T cell priming not only in draining sites, but also in the genital and intestinal tracts and in the spleen. (C) 2009 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available