Journal
VACCINE
Volume 27, Issue 20, Pages 2712-2722Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.02.027
Keywords
Influenza vaccine production; MDCK; Apoptosis; Flow cytometry; Mathematical modeling; Wisconsin; Puerto Rico; Virus-host cell interaction
Categories
Ask authors/readers for more resources
Cell culture-based influenza vaccine manufacturing is of growing importance. Depending on virus strains, differences in infection dynamics, virus-induced apoptosis, cell lysis and virus yields are observed. Comparatively little is known concerning details of virus-host cell interaction on a cellular level and virus spreading in a population of cells in bioreactors. In this study, the infection of MDCK cells with different influenza A virus strains in lab-scale microcarrier culture was investigated by flow cytometry. Together with the infection status of cells, virus-induced apoptosis was monitored. A mathematical model has been formulated to describe changes in the concentration of uninfected and infected adherent cells, dynamics of virus particle release (infectious virions, hemgglutinin content), and the time course of the percentage composition of the cell population. (C) 2009 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available