Journal
VACCINE
Volume 27, Issue 34, Pages 4662-4671Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.05.094
Keywords
Adjuvant; Innate defence regulator peptide; Host defense peptide; CpG DNA; Oligodeoxynucleotides; Toll-like receptors; Formulation
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Funding
- Global Health Research Initiative
- Canadian Cystic Fibrosis Foundation
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There has been an increased demand for the development of novel vaccine adjuvants that lead to enhanced induction of protection from infectious challenges and development of immunological memory. A novel vaccine adjuvant was developed comprising a complex containing CpG oligonucleotide and the synthetic cationic innate defence regulator peptide HH2 that has enhanced immune modulating activities. The complex of HH2 and the CpG oligonucleotide 10101 was a potent inducer of cytokine/chemokine expression ex vivo, retained activity following extended storage, had low associated cytotoxicity, and upregulated surface marker expression in dendritic cells, a critical activity for a vaccine adjuvant. Immunization of mice with a coformulation of the HH2-CpG complex and pertussis toxoid significantly enhanced the induction of toxoid-specific antibody titres when compared to toxoid alone, inducing high titres of IgG1 and IgG2a, typical of a balanced Th1/Th2 response, and also led to high IgA titres. This study demonstrates the potential application of the HH2-CpG complex as a vaccine adjuvant. (C) 2009 Elsevier Ltd. All rights reserved.
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