Journal
VACCINE
Volume 27, Issue 10, Pages 1571-1578Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.01.002
Keywords
Edwardsiella tarda; Bacterial ghosts; Oral vaccination; Cellular immunity; Humoral immunity
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Bacterial ghosts may be generated by the controlled expression of the phiX174 lysis gene E in Gram-negative bacteria and they are intriguing vaccine candidates since ghosts retain functional antigenic cellular determinants often lost during traditional inactivation procedures. The Edwardsiella tarda ghost (ETG) vaccine was prepared using this technology and tested in vaccination trials. Control groups included mice immunized with formalin-killed E tarda (FKC) or mice treated with phosphate-buffered saline (PBS), respectively. The results showed that serum IgA and IgG antibody titers were significantly higher in the ETG-vaccinated group compared to the other groups. In addition, CD8+ T cell counts in peripheral blood were elevated in the ETG groups. Most important, ETG-immunized mice were significantly protected against E. tarda challenge (86.7% survival) compared to 73.3 and 33.3% survival in the FKC-immunized and PBS-treated control, respectively, Suggesting that an ETG oral vaccine could confer protection against infection in a mouse model of disease. (C) 2009 Elsevier Ltd. All rights reserved.
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