Journal
VACCINE
Volume 27, Issue 4, Pages 573-580Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.10.090
Keywords
Toxoplasma gondii; Mice; Eye; Vaccine
Categories
Funding
- NIH [A119613]
- Guangdong Provincial Natural Science Foundation of China [06021302]
- Returned Overseas Chinese Scholars
- State Education Ministry, China [20061331]
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The risk of blindness Caused by ocular toxoplasmosis Supports efforts to improve Our understanding for control of this disease. In this Study, the involvement of CD8(+), CD4(+), B cell, and IL-10 gene in the immune response of primary ocular infection with the temperature-sensitive mutant (ts-4) of the RH Toxoplasma gondii strain, and in the protective immunity of ocular ts-4 vaccination and challenge with RH strain was investigated ill murine models utilizing inbred C57BL/6 mice-deficient in CD4(+), CD8(+), B cells (mu MT), or IL-10 gene. Compared to naive mice, all WT and mutant mice had different degree of ocular pathological changes after ts-4 ocular infection, in which both CD8 KO and IL-10 KO mice showed the most severe ocular lesions. Immunized by ts-4 intracameral (i.c.) inoculation, all Mutant mice had partially decreased vaccine-induced resistance associated with increased ocular parasite burdens after RH strain challenge. A significant increase of the percentages of B cells and CD8(+) T cells in the draining lymph nodes were observed in WT and IL-10 KO mice after either infection or challenge. The levels of specific anti-toxoplasma IgG in both eye fluid and serum from all the mice were significantly increased after ts-4 i.c. immunization, except mu MT mice. These results suggest that the avirulent ts-4 of T. gondii inoculated intracamerally can induce both ocular pathology and ocular protective immunity: CD4(+), CD8(+), B cell, and IL-10 gene are all necessary to the vaccine-induced resistance to ocular challenge by virulent RH strain, ill which CD8(+) T cells are the most important component. (C) 2008 Elsevier Ltd. All rights reserved.
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