Journal
VACCINE
Volume 27, Issue 43, Pages 5998-6003Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2009.07.079
Keywords
Pseudoparticle neutralization assay; Avian influenza virus; H5N1; Pandemic vaccine; Cross-clade neutralization; Serological methods
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Funding
- National Institute of Hygiene and Epidemiology (NIHE) [VietNam/1194/04HA]
- Medical Research Council [G0600369] Funding Source: researchfish
- MRC [G0600369] Funding Source: UKRI
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The standard serological methods present limitations for the measurement of immunity against H5N1 influenza strains. The hemagglutination inhibition (HI) assay lacks sensitivity and requires standardization, while the viral micro-neutralization (MN) assay needs handling of live virus. We produced pseudoparticles expressing hemagglutinin from clades 1 or 2 H5N1 in order to measure neutralizing antibodies in human sera after prime-boost vaccination with plain or MF59-adjuvanted H5N1 clade 1 subunit vaccines. Titers measured by pseudoparticle neutralization (PPN) assay significantly correlated with those measured by HI, single radial haemolysis or MN, with a PPN titer of 1:357 corresponding to an MN titer of 1:80. Notably, results from the PPN assay, confirm that MF59-H5N1 vaccine induces potent and long-lasting neutralizing antibody responses not only against the vaccine strain, but also against several heterologous clade 2 strains. Overall, the PPN assay represents a valid alternative to conventional serological methods for the evaluation of H5N1 vaccine immunogenicity. (C) 2009 Elsevier Ltd. All rights reserved
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