Journal
VACCINE
Volume 26, Issue 46, Pages 5888-5895Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.05.095
Keywords
Canine visceral leishmaniasis; Recombinant vaccine; A2 antigen
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Funding
- Millennium Institute for Vaccine Development and Technology (CNPq) [420067/2005-1]
- FAPEMIG
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In this study, we investigated in dogs the immunogenicity and protective immunity against Leishmania (Leishmania) chagasi infection induced by vaccination with a formulation containing the recombinant A2 protein, an amastigote specific antigen, and saponin. Vaccinated animals produced significantly increased levels of total IgG and IgG2, but not lgG1 anti-A2 antibodies, and remained negative in conventional leishmaniasis serodiagnostic methods. Significantly increased IFN-gamma and low IL-10 levels were detected in vaccinated animals before and after challenge, as compared to control animals. Importantly, while the symptoms onset appeared as early as three months after infection in most control dogs, 14 months after challenge, 5 out of 7 vaccinated dogs remained asymptomatic. Therefore, immunization with rA2 antigen was immunogenic and induced partial protection in dogs, and allowed the serological differentiation between vaccinated and infected animals, an important requirement for a canine visceral leishmaniasis (CVL) vaccine. (C) 2008 Elsevier Ltd. All rights reserved.
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