Journal
VACCINE
Volume 26, Issue 33, Pages 4194-4203Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.05.079
Keywords
Clostrdium perfringens; vaccine; necrotic enteritis; chickens
Categories
Funding
- Ontario Ministry of Agriculture, Food, and Rural Affairs
- Poultry Industry Council, Ont., Canada
- Natural Sciences and Engineering Research Council of Canada
- Agriculture and Agri-Food Canada
- Canadian Poultry Research Council of Canada
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Necrotic enteritis (NE) in broiler chickens is caused by Clostridium perfringens but currently no effective vaccine is available. Our previous study showed that certain C. perfringens secreted proteins when administered intramuscularly protected chickens against experimental infection. In the current study, genes encoding three C perfringens proteins: fructose-biphosphate-aldolase (FBA), pyruvate:ferredoxinoxidoreductase (PFOR) and hypothetical protein (HP), were cloned into an avirulent Salmonella enterica sv. typhimurium vaccine vector. Broiler chickens immunized orally with recombinant Salmonella expressing FBA or HP proteins were significantly protected against NE challenge. Immunized birds developed serum and mucosal antibodies to both clostridial and Salmonella antigens. This study showed the oral immunizing ability of two C. perfringens antigens against NE in broiler chickens through an attenuated Salmonella vaccine vector. (C) 2008 Elsevier Ltd. All rights reserved.
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