Journal
VACCINE
Volume 26, Issue 37, Pages 4813-4818Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2008.06.102
Keywords
soluble Leishmania donovani (SLD) promastigotes; Th1-stimulatory proteins; cured Leishmania patients/hamsters; LTT; cytokines
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Funding
- DBT, New Delhi
- CSIR, New Delhi
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Earlier, we have identified soluble antigenic fraction ranging from 68 to 97.4 kDa (F2-fraction) of Leishmania donovani promastigote, which induced Th1-immunostimulatory cellular responses in both cured Leishmania patients/hamsters and exhibited significant prophylactic potential against experimental visceral leishmaniasis (VL). In the present study, we have further fractionated F2-fraction by continuous elution SDS-PAGE and subjected them to re-evaluation for their ability to induce cellular responses. Out of seven sub-fractions: F2.1-F2.7, only four F2.4-F2.7 (89.9-97.1 kDa), individually or in pooled subfractions (P4-7), stimulated Th1-type remarkable lymphoproliferative, NO, IFN-gamma and IL-12 responses in Leishmania-infected cured/exposed patients and hamsters. Interestingly, optimum Th1-responses were obtained with P4-7 suggesting the presence of immunostimulatory molecules in it and may be exploited for developing a successful subunit vaccine against VL. (C) 2008 Elsevier Ltd. All rights reserved.
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