Journal
ULTRASOUND IN MEDICINE AND BIOLOGY
Volume 40, Issue 1, Pages 138-147Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2013.08.018
Keywords
Laser obscuration/extinction; Flow cytometry; Electro-impedance sensing; Generalized Lorenz-Mie theory; Lipid-coated microbubbles; Polystyrene beads
Funding
- NSF [CBET-1133687, CBET-1059726]
- Directorate For Engineering [1133687] Funding Source: National Science Foundation
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Single-particle optical sizing techniques are being used to determine the size distributions of microbubble ultrasound contrast agents and to study the dynamics of individual microbubbles during ultrasound stimulation. The goal of this study was to compare experimental light obscuration and scattering measurements of microbubble size distributions with predictions from generalized Lorenz-Mie scattering theory (GLMT). First, we illustrate that a mono-modal size distribution can be misrepresented by single-particle light obscuration measurements as multi-modal peaks because of non-linearities in the extinction cross section-versus-diameter curve. Next, polymer bead standards are measured to provide conversion factors between GLMT calculations and experimental flow cytometry scatter plots. GLMT calculations with these conversion factors accurately predict the characteristic Lissajous-like serpentine scattering plot measured by flow cytometry for microbubbles. We conclude that GLMT calculations can be combined with optical forward and side scatter measurements to accurately determine microbubble size. (C) 2014 World Federation for Ultrasound in Medicine & Biology.
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