Journal
ULTRASOUND IN MEDICINE AND BIOLOGY
Volume 39, Issue 11, Pages 2147-2157Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2013.05.016
Keywords
Protamine; Cationic nanobubble; Gene transfection; Contrast-enhanced ultrasound
Funding
- Natural Science Foundation of China (NSFC) [30970830]
- Natural Science Foundation of Chongqing City [cstc2012jjB10072]
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In this study, we aimed to prepare a novel type of microbubble (MB), protamine cationic nanobubble (NB), to provide a new vector for tumor gene therapy. We prepared cationic NBs with protamine and other lipid components using mechanical oscillation. The protamine cationic NBs had a mean diameter of 521.2 +/- 37.57 nm, a zeta potential of +18.5 mV, and a gene-carrying capacity of 15.69 mu g androgen receptor (AR) siRNA per 10(8) NBs. The cationic NBs exhibited superior contrast enhancement for in vivo imaging compared with SonoVue (Bracco, Geneva, Switzerland), and their physical properties did not change significantly after 1 wk; meanwhile, the transfection efficiency of the cationic NBs in androgen-independent prostate cancer cells mediated by ultrasound irradiation was better than that of liposomes (82.17 +/- 7.4% vs. 55.04 +/- 5.4%, p < 0.01). Therefore, the protamine cationic NB can be considered for use as a novel type of gene-loading MB for ultrasound imaging and MB-mediated gene therapy of tumors. (E-mail: guoyanli71@aliyun.com) (C) 2013 World Federation for Ultrasound in Medicine & Biology.
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