Journal
ULTRASOUND IN MEDICINE AND BIOLOGY
Volume 37, Issue 2, Pages 321-330Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.ultrasmedbio.2010.10.019
Keywords
Embryonic stem cells; Embryoid bodies; Gene expression; Differentiation; Neural; Pluripotency; Ultrasound; Cell manipulation; Microenvironment
Funding
- EU [MTKD-CT-2006- 042519 - Nanofab]
- Royal Society [2006/R2]
- Trinity College Dublin
- Wellcome Trust [089877/Z/09/Z]
- HRB [H01242]
- HRB/Fighting Blindness [T01060]
- Wellcome Trust [089877/Z/09/Z] Funding Source: Wellcome Trust
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In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine. (E-mail: Bazoud@tcd.ie) (C) 2011 World Federation for Ultrasound in Medicine & Biology.
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