Journal
ULTRASONICS SONOCHEMISTRY
Volume 20, Issue 6, Pages 1390-1400Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2013.04.005
Keywords
Ultrasonic horn reactor; Ultrasonic bath reactor; Defatted wheat germ; Protein structure; ACE-inhibitory activity
Categories
Funding
- National Natural Science Foundation of China [31071502]
- National High Technology Research and Development Program 863 [2013AA102203]
- Special Fund for Agro-scientific Research in the Public Interest [201303071]
- Natural Science Foundation of Zhejiang Province of China [Y3110025]
- Key Laboratory for Physical Processing of Agricultural Products [JAPP2010-4]
- Jiangsu Government Scholarship Fund
- Foundation for the Eminent Talents and Research Foundation for Advanced Talents of Jiangsu University [12JDG074]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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The ultrasonic horn and bath reactors were compared based on production of angiotensin-converting-enzyme (ACE) inhibitory peptides from defatted wheat germ proteins (DWGP). The DWGP was sonicated before hydrolysis by Alcalase. Degree of hydrolysis, ACE-inhibitory activity, surface hydrophobicity, fluorescence intensity, free sulfhydryl (SH), and disulfide bond (SS) were determined. The highest ACE-inhibitory activity of DWGP hydrolysate was obtained at power intensity of 191.1 W/cm(2) for 10 min in the ultrasonic horn reactor. The fixed frequency of 33 kHz and the sweep frequency of 40 +/- 2 kHz resulted in the maximum ACE-inhibitory activity. The combined irradiation of dual fixed frequency (24/68 kHz) produced significant increase in ACE-inhibitory activity compared with single frequency (33 kHz). The ultrasonic probe resulted in significant higher ACE-inhibitory activity compared with ultrasonic bath operating at single or dual fixed and sweep frequencies. The changes in conformation of the DWGP due to sonication were confirmed by the changes in fluorescence intensity, surface hydrophobicity, SHf and SS contents and they were found in conformity with the ACE-inhibitory activity in case of the ultrasonic horn reactor but not in bath reactor. (C) 2013 Elsevier B.V. All rights reserved.
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