Journal
ULTRASONICS SONOCHEMISTRY
Volume 19, Issue 5, Pages 1021-1026Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2012.02.006
Keywords
Wheat germ protein; ACE inhibitory peptide; Ultrasound-assisted enzymolysis; ACE inhibitory activity; Kinetics
Categories
Funding
- Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions
- Jiangsu University [10JDG121]
- Postdoctoral Funds of Jiangsu Province [1101039C]
- National Natural Science Foundation of China [31071502]
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There is a great demand for developing efficient enzymolysis methods in order to increase the enzymolysis efficiencies and activities of angiotensin converting enzyme (ACE) inhibitory peptides from wheat germ protein. The enzymolysis kinetics, ACE inhibitory activity of peptide and conversion rate of protein were studied using sweep frequency and pulsed (SFP) ultrasound-assisted enzymolysis and the results were compared with traditional enzymolysis. The studied factors were enzymolysis time and substrate concentration. By considering the activity of ACE inhibitory peptide and operation cost, the recommended conditions of SFP ultrasound-assisted enzymolysis were enzymolysis time of 120 min and substrate concentration of 24.0 g/L, which gave high conversion rates of protein (60.7%) and ACE inhibitory activity of peptide (65.9%). Compared to traditional enzymolysis, SFP ultrasound-assisted enzymolysis significantly increased the initial reaction rate (V) by 60.0% at substrate concentration of 24.0 g/L, increased the apparent breakdown rate constant (k(A)) by 66.7%, decreased the apparent constant (K-M) by 6.9%, and raised the conversion rate of protein by 35.5% and ACE inhibitory activity of peptides by 35.6% under the recommended conditions. It has been concluded that SFP ultrasound can remarkably raise the enzymolysis efficiency and activity of ACE inhibitory peptides from wheat germ protein. (C) 2012 Elsevier B.V. All rights reserved.
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