Journal
ULTRASONICS SONOCHEMISTRY
Volume 19, Issue 1, Pages 198-208Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultsonch.2011.05.010
Keywords
Microbubble; Ultrasound; Contrast agent; Production; Sonication; Cavitation
Categories
Funding
- NIH [R01CA99178, R37EB002641, R01HL82481]
Ask authors/readers for more resources
Uniformly-sized preparations with average microbubble (MB) diameters from 1 to 7 mu m were produced reliably by sonicating decafluorobutane-saturated solutions of serum albumin and dextrose. Detailed protocols for producing and size-separating the MBs are presented, along with the effects that changing each production parameter (serum albumin concentration, sonication power, sonication time, etc.) had on MB size distribution and acoustic stability. These protocols can be used to produce MBs for experimental applications or serve as templates for developing new protocols that yield MBs with physical and acoustic properties better suited to specific applications. Size stability and ultrasonic performance quality control tests were developed to assure that successive MB preparations perform identically and to distinguish the physical and acoustic properties of identically sized MBs produced with different serum albumin-dextrose formulations and sonication parameters. MBs can be stored at 5 degrees C for protracted periods (2 weeks to one year depending on formulation). (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available