Journal
ULTRASONICS
Volume 54, Issue 6, Pages 1656-1662Publisher
ELSEVIER
DOI: 10.1016/j.ultras.2014.03.003
Keywords
Tandem shock waves; Acoustic cavitation; Genetic transformation; Filamentous fungi; Microjets
Funding
- Consejo Nacional de Ciencia y Tecnologia (CONACYT) [219950, 219893]
- Universidad Nacional Autonoma de Mexico (UNAM) [DGAPA IN108410-3]
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Filamentous fungi are used in several industries and in academia to produce antibiotics, metabolites, proteins and pharmaceutical compounds. The development of valuable strains usually requires the insertion of recombinant deoxyribonucleic acid; however, the protocols to transfer DNA to fungal cells are highly inefficient. Recently, underwater shock waves were successfully used to genetically transform filamentous fungi. The purpose of this research was to demonstrate that the efficiency of transformation can be improved significantly by enhancing acoustic cavitation using tandem (dual-pulse) shock waves. Results revealed that tandem pressure pulses, generated at a delay of 300 mu s, increased the transformation efficiency of Aspergillus niger up to 84% in comparison with conventional (single-pulse) shock waves. This methodology may also be useful to obtain new strains required in basic research and biotechnology. (C) 2014 Elsevier B.V. All rights reserved.
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