Journal
ULTRAMICROSCOPY
Volume 110, Issue 6, Pages 689-693Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ultramic.2010.02.033
Keywords
Immunostimulatory DNA; Atomic force microscopy; G-quadruplex; TLR
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Immunostimulatory CpG-DNA activates the innate immune system by binding to Toll-like receptor 9. Structurally different CpG-containing oligonucleotides trigger a different type of immune response while activating the same receptor. We therefore investigated the higher order structure of two different classes of immunostimulatory CpG-DNA Class A, which contains a partly self-complementary sequence and poly-G ends, forms duplexes and nanoparticles in salt solution, while class B, which does not contain these features and is purely linear, does not form a duplex or nanoparticles. Results obtained here by high-resolution atomic force microscopy of classes A and B CpG-DNA, reflect these differences in secondary structure. Detailed structural analysis of the atomic force microscopy topographs is presented for two different sample preparation methods. (C) 2010 Elsevier B.V. All rights reserved.
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