Journal
ULTRAMICROSCOPY
Volume 108, Issue 11, Pages 1478-1483Publisher
ELSEVIER
DOI: 10.1016/j.ultramic.2008.03.014
Keywords
Cryo-TEM sample preparation; Glove-box; Vitrobot; Langmuir films; Mineralization at interfaces; DNA adsorption at interfaces; Time resolved cryo-TEM
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Aqueous interfaces are of paramount importance in the study of biological systems as well as in the biomedical sciences. To study these interfaces at the nanometer level it is of interest to develop methods that allow their observation with cryogenic transmission electron microscopy (cryo-TEM). Prevention of dehydration to preserve the native state during sample preparation prior to vitrification is often one of the most important parameters to control in cryo-TEM experiments. For the preparation of these types of samples, we felt the need for an extended workspace with temperature and humidity control; a 'glove-box' that seamlessly connects to the vitrification instrument, the Vitrobot (TM). In this paper we describe the use of the glove-box in the 2D and 3D cryo-TEM study of DNA adsorption and calcium carbonate mineralization to Langmuir films. The data presented illustrates the necessity of a humidity-controlled environment to preserve the original native state of the monolayer system (C) 2008 Elsevier B.V. All rights reserved.
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