4.1 Article

Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.)

Journal

TURKISH JOURNAL OF BIOLOGY
Volume 38, Issue 5, Pages 593-600

Publisher

Tubitak Scientific & Technological Research Council Turkey
DOI: 10.3906/biy-1403-29

Keywords

Carrot; androgenesis; putrescine; spermidine; regeneration

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Funding

  1. National Centre for Research and Development [N R12001106]

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This study examines the influence of the polyamines putrescine and spermidine on the efficiency of androgenesis in anther cultures of 2 carrot cultivars, Kazan F-1 and Narbonne F-1, and the effect of putrescine on the process of plant regeneration from androgenetic embryos. In the Kazan F-1 variety, an increase in the number of obtained embryos was achieved using each of the 2 polyamines separately and in combination. In contrast, no beneficial effects of polyamines on the efficiency of androgenesis were observed in the Narbonne F-1 variety. Putrescine added to the regeneration medium increased the number of obtained plants. For embryos obtained on induction medium without putrescine, the best concentration was 160 mg putrescine in 1 L of regeneration medium, and for those obtained on the induction medium with putrescine the best concentration was 0.5 mg putrescine per 1 L. All of the resulting plants, both in the experimental and control combinations, had a doubled set of chromosomes. The vast majority of them were homozygous for both isoenzymes, glucose-6-phosphate isomerase and aspartate aminotransferase, and the distribution of homo-and heterozygous received combinations with the polyamines and in the control was very similar.

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