Journal
TUMOR BIOLOGY
Volume 36, Issue 2, Pages 921-928Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-014-2719-x
Keywords
miR-152; KIT; Liver cancer stem cells; Proliferation
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Funding
- Science and Technology Program of Guangdong Province [2008B030301028]
- Science and Technology Innovation Fund of Guangdong Medical College [STI F201107]
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miR152 is involved in diverse biological functions and development of disease. This study investigates the role of mir-152 in cell proliferation and colony formation of liver cancer stem cells. We show that exogenous overexpression of mir-152 suppresses cell proliferation and colony formation in CD133(+) hep3B cells. We also show that KIT is a direct target of miR-152 and miR-152 downregulates protein expression of KIT by directly binding to 3' untranslated region of KIT. Downregulation of KIT by specific siRNAs inhibits proliferation and colony formation of CD133(+) hep3B cells, which is similar to inhibitory effects of miR-152. Moreover, exogenous expression of KIT compromises inhibitory effects of miR-152 on cell proliferation and colony formation. Our findings suggest that mir-152 inhibits cell proliferation and colony formation of CD133(+) hep3B cells by targeting KIT.
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