Journal
TUMOR BIOLOGY
Volume 36, Issue 4, Pages 3025-3033Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-014-2937-2
Keywords
Oral precancer; Cancer; similar to 5 kb deletion; D-loop mutation
Categories
Funding
- Indian Statistical Institute
Ask authors/readers for more resources
Apart from genomic DNA, mutations at mitochondrial DNA (mtDNA) have been hypothesized to play vital roles in cancer development. In this study, similar to 5 kb deletion and D-loop mutations in mtDNA and alteration in mtDNA content were investigated in buccal smears from 104 healthy controls and 74 leukoplakia and 117 cancer tissue samples using Taqman-based quantitative assay and re-sequencing. The similar to 5 kb deletion in mtDNA was significantly less (9.8 and 10.5 folds, P<0.0001) in cancer tissues compared to control and leukoplakia tissues, respectively. On the other hand, somatic mutations in D-loop, investigated in 54 controls, 50 leukoplakias and 56 cancer patients, were found to be significantly more in cancer tissues, but not in leukoplakia tissues, compared to control (Z-score=5.4). MtDNA contents were observed to be significantly more in leukoplakia (2.1 folds, P=0.004) and cancer (1.6 folds, P=0.03) tissues compared to control tissues. So, D-loop somatic mutations and similar to 5 kb deletion patterns could be used as distinguishing markers between precancer and cancer tissues. This observation further suggests that somatic mutations in D-loop may facilitate carcinogenesis and cancer cells with less similar to 5 kb deletion, i.e., intact mtDNA, may become resistant to apoptosis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available